Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # GJB1/1753

Concentration

0.2 mg/ml

View all Connexin 32/GJB1 Antibodies »

Product Summary for Connexin 32/GJB1 Antibody (GJB1/1753)

Immunogen

Recombinant human Connexin 32/GJB1 protein (Uniprot: P08034)

Localization

Cytoplasmic and Cell Surface

Specificity

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54359)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Connexin 32/GJB1 Antibody (GJB1/1753)

Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384]

Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384]

Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384] - Formalin-fixed, paraffin-embedded human Tonsil stained with Connexin 32 Monoclonal Antibody (GJB1/1753)
Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384]

Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384]

Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384] - Formalin-fixed, paraffin-embedded human Liver stained with Connexin 32 Monoclonal Antibody (GJB1/1753)

Applications for Connexin 32/GJB1 Antibody (GJB1/1753)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Connexin 32/GJB1

Gap junctions are aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemichannels, called connexons, from both participating cells. These channels are permeate to molecules as large as 1 kD, and they have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which are termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins ranging between molecular weights of 26 and 70 kD. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group to which Cx26, Cx30, Cx31, Cx31.1 and Cx32 belong, and the other termed class II or alpha group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46.

Alternate Names

CMTX1, CX32, GJB1

Gene Symbol

GJB1

UniProt

P08034 (Human)

Additional Connexin 32/GJB1 Products

Product Documents for Connexin 32/GJB1 Antibody (GJB1/1753)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Connexin 32/GJB1 Antibody (GJB1/1753)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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