Connexin 32/GJB1 Antibody (SR1532)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22393

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22393-100ul
NBP3-22393-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1532

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Connexin 32/GJB1 Antibodies »

Product Summary for Connexin 32/GJB1 Antibody (SR1532)

Immunogen

A synthesized peptide derived from human Connexin 32/GJB1 (Uniprot #: P08034)

Specificity

Detects endogenous levels of total Connexin 32/GJB1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Connexin 32/GJB1 Antibody (SR1532)

Western Blot: Connexin 32/GJB1 Antibody (SR1532) [NBP3-22393] -

Western Blot: Connexin 32/GJB1 Antibody (SR1532) [NBP3-22393] - Western blot analysis using NBP3-22393 in MCF-7 cell lysate.

Applications for Connexin 32/GJB1 Antibody (SR1532)

Application
Recommended Usage

Flow Cytometry

1:50

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Connexin 32/GJB1

Gap junctions are aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemichannels, called connexons, from both participating cells. These channels are permeate to molecules as large as 1 kD, and they have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which are termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins ranging between molecular weights of 26 and 70 kD. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group to which Cx26, Cx30, Cx31, Cx31.1 and Cx32 belong, and the other termed class II or alpha group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46.

Alternate Names

CMTX1, CX32, GJB1

Gene Symbol

GJB1

Additional Connexin 32/GJB1 Products

Product Documents for Connexin 32/GJB1 Antibody (SR1532)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Connexin 32/GJB1 Antibody (SR1532)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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