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Connexin 32/GJB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59139

Novus Biologicals, part of Bio-Techne
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NBP1-59139

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Summary for Connexin 32/GJB1 Antibody

Immunogen

Synthetic peptides corresponding to GJB1 (gap junction protein, beta 1, 32kDa) The peptide sequence was selected from the C terminal of GJB1. Peptide sequence GFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Connexin 32/GJB1 Antibody

Western Blot: Connexin 32/GJB1 Antibody [NBP1-59139]

Western Blot: Connexin 32/GJB1 Antibody [NBP1-59139]

Western Blot: GJB1 Antibody [NBP1-59139] - Titration: 2.5ug/ml, Positive Control: Jurkat cell lysate.
Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody [NBP1-59139]

Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody [NBP1-59139]

Immunohistochemistry-Paraffin: GJB1 Antibody [NBP1-59139] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.

Applications for Connexin 32/GJB1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Connexin 32/GJB1

This gene encodes for a Connexin 32 protein. A large Charcot-Marie-Tooth disease family has been identified with a novel mutation in the Cx32 P2 promoter region at position -526bp. Cx32 mutants that are associated with a CNS phenotype may have toxic effects in oligodendrocytes.

Alternate Names

CMTX1, CX32, GJB1

Gene Symbol

GJB1

UniProt

Additional Connexin 32/GJB1 Products

Product Documents for Connexin 32/GJB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Connexin 32/GJB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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