Connexin 45/GJC1 Antibody (5B9.2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29754
![Immunocytochemistry/ Immunofluorescence: Connexin 45/GJC1 Antibody (5B9.2) - BSA Free [NBP2-29754]](https://resources.bio-techne.com/images/products/Connexin-45-GJC1-Antibody-5B9-2-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-29754-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Connexin 45/GJC1 Antibody (5B9.2) - BSA Free [NBP2-29754]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat, Rabbit
Predicted:
Canine (100%), Mouse (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5B9.2
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Connexin 45/GJC1 Antibody (5B9.2) - BSA Free
Immunogen
The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-377 of human connexin 45.
Reactivity Notes
Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Connexin 45/GJC1 Antibody (5B9.2) - BSA Free
Immunocytochemistry/ Immunofluorescence: Connexin 45/GJC1 Antibody (5B9.2) - BSA Free [NBP2-29754]
Immunocytochemistry/Immunofluorescence: Connexin 45/GJC1 Antibody (5B9.2) - BSA Free [NBP2-29754] - Mouse anti-Connexin 45 staining of Cx45 transfected rat C6 cells.Applications for Connexin 45/GJC1 Antibody (5B9.2) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:100-1:2000
Application Notes
ImmunBlotting (~46 kDa)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
20mM Phosphate (pH 7.6) and 0.25M NaCl
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Connexin 45/GJC1
Additional Connexin 45/GJC1 Products
Product Documents for Connexin 45/GJC1 Antibody (5B9.2) - BSA Free
Product Specific Notices for Connexin 45/GJC1 Antibody (5B9.2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...