Cortactin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-855

Novus Biologicals, part of Bio-Techne
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NB100-855
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cortactin Antibodies »

Product Specifications

Immunogen

Peptide with sequence WKASAGHAVSIA-C corresponding to N-Terminus according to NP_005222.2, NP_612632.1.

Localization

Cytoplasmic

Specificity

This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cortactin Antibody

Western Blot: Cortactin Antibody [NB100-855]

Western Blot: Cortactin Antibody [NB100-855]

Western Blot: Cortactin Antibody [NB100-855] - Analysis of Cortactin in Mouse Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Cortactin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in mouse and rat brain lysates (calculated MW of 61.6 kDa band according to human NP_005222.2 and of 61.2 kDa band according to mouse NP_031829.2). The observed molecular weights correspond to earlier findings is reported in scientific literature with different antibodies (PMID 18495841).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cortactin

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.

Alternate Names

Amplaxin, CTTN, EMS1

Entrez Gene IDs

2017 (Human); 13043 (Mouse)

Gene Symbol

CTTN

UniProt

NP_005222. (Human)

Additional Cortactin Products

Product Documents for Cortactin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cortactin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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