COUP-TF I/NR2F1 Antibody (JE31-95)

Western Blot: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211]

Western Blot: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211] - Western blot analysis of COUP-TF I/NR2F1 on different lysates with Rabbit anti-COUP-TF I/NR2F1 antibody (NBP3-32211) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (10 ug/Lane)
Lane 2: Human brain tissue lysate (40 ug/Lane)

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 8 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32211) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211]

Immunohistochemistry: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-COUP-TF I/NR2F1 antibody (NBP3-32211) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32211) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.