COX-1 Antibody (6B6)

Western Blot: COX-1 Antibody (6B6) [NBP3-26362] -

Western Blot: COX-1 Antibody (6B6) [NBP3-26362] - Positive Western Blot detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue
All lanes: COX-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 69, 65, 62, 57, 72, 73 kDa
Observed band size: 72 kDa

Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] -

Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] - Overlay histogram showing Hela cells stained with COX-1 Antibody (6B6) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.