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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # COX2/3320R

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide corresponding to COX-2 residues within aa504-604 of COX-2 (Uniprot: P35354)

Localization

Cytoplasm, Cell surface

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07256).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for COX-2 Antibody (COX2/3320R) - Azide and BSA Free

Immunohistochemistry-Paraffin: COX-2 Antibody (COX2/3320R) - Azide and BSA Free [NBP3-08301]

Immunohistochemistry-Paraffin: COX-2 Antibody (COX2/3320R) - Azide and BSA Free [NBP3-08301]

Immunohistochemistry-Paraffin: COX-2 Antibody (COX2/3320R) - Azide and BSA Free [NBP3-08301] - Formalin-fixed, paraffin-embedded human kidney stained with COX-2 Recombinant Rabbit Monoclonal Antibody (COX2/3320R).
Immunohistochemistry-Paraffin: COX-2 Antibody (COX2/3320R) - Azide and BSA Free [NBP3-08301]

Immunohistochemistry-Paraffin: COX-2 Antibody (COX2/3320R) - Azide and BSA Free [NBP3-08301]

Immunohistochemistry-Paraffin: COX-2 Antibody (COX2/3320R) - Azide and BSA Free [NBP3-08301] - Formalin-fixed, paraffin-embedded human kidney stained with COX-2 Recombinant Rabbit Monoclonal Antibody (COX2/3320R).

Applications for COX-2 Antibody (COX2/3320R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Gene Symbol

PTGS2

Additional COX-2 Products

Product Documents for COX-2 Antibody (COX2/3320R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COX-2 Antibody (COX2/3320R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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