COX4 Antibody (3P8J2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15416
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3P8J2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for COX4 Antibody (3P8J2)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human COX4 (P13073). MLATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEW
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COX4 Antibody (3P8J2)
Western Blot: COX4 Antibody (3P8J2) [NBP3-15416]
Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.Immunohistochemistry-Paraffin: COX4 Antibody (3P8J2) [NBP3-15416]
Immunohistochemistry-Paraffin: COX4 Antibody (9X9I0) [NBP3-15416] - Immunohistochemistry of paraffin-embedded human colon carcinoma using COX4 Rabbit mAb (NBP3-15416) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for COX4 Antibody (3P8J2)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: COX4
Long Name
Cytochrome c Oxidase Subunit IV
Alternate Names
COX4I2
Gene Symbol
COX4I1
Additional COX4 Products
Product Documents for COX4 Antibody (3P8J2)
Product Specific Notices for COX4 Antibody (3P8J2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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