COX5A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06072
![Western Blot: COX5A Antibody [NBP3-06072]](https://resources.bio-techne.com/images/products/COX5A-Antibody-Western-Blot-NBP3-06072-img0001.jpg "Western Blot: COX5A Antibody [NBP3-06072]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for COX5A Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human COX5A fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for COX5A Antibody
Western Blot: COX5A Antibody [NBP3-06072]
Western Blot: COX5A Antibody [NBP3-06072] - Anti-COX5A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:15 kDa (We are unsure as to the identity of these extra bands.)![Immunoprecipitation: COX5A Antibody [NBP3-06072]](https://resources.bio-techne.com/images/products/COX5A-Antibody-Immunoprecipitation-NBP3-06072-img0002.jpg "Immunoprecipitation: COX5A Antibody [NBP3-06072]")
Immunoprecipitation: COX5A Antibody [NBP3-06072]
Immunoprecipitation: COX5A Antibody [NBP3-06072] - COX5A was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-COX5A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-COX5A rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :13 kDaApplications for COX5A Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COX5A
Alternate Names
COX, COX-VA, Cytochrome c oxidase polypeptide Va, cytochrome c oxidase polypeptide, mitochondrial, cytochrome c oxidase subunit 5A, mitochondrial, cytochrome c oxidase subunit Va, mitochondrial cytochrome c oxidase subunit Va, VA
Gene Symbol
COX5A
Additional COX5A Products
Product Specific Notices for COX5A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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