COX5b Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92927
![Immunohistochemistry-Paraffin: COX5b Antibody - BSA Free [NBP2-92927]](https://resources.bio-techne.com/images/products/COX5b-Antibody-Immunohistochemistry-Paraffin-NBP2-92927-img0008.jpg "Immunohistochemistry-Paraffin: COX5b Antibody - BSA Free [NBP2-92927]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2). ASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVPQQLAH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for COX5b Antibody - BSA Free
Western Blot: COX5b Antibody [NBP2-92927] -
Western Blot: COX5b Antibody [NBP2-92927] - analysis of various lysates, using COX5B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: COX5b Antibody - BSA Free [NBP2-92927] -
Immunocytochemistry/ Immunofluorescence: COX5b Antibody - BSA Free [NBP2-92927] - Immunofluorescence analysis of PC-12 cells using COX5b Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: COX5b Antibody - BSA Free [NBP2-92927] -
Immunocytochemistry/ Immunofluorescence: COX5b Antibody - BSA Free [NBP2-92927] - Immunofluorescence analysis of NIH/3T3 cells using COX5b Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Applications for COX5b Antibody - BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:50-1:100
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: COX5b
Alternate Names
COXVB, Cytochrome c oxidase polypeptide Vb, cytochrome c oxidase polypeptide VB, mitochondrial, cytochrome c oxidase subunit 5B, mitochondrial, cytochrome c oxidase subunit Vb
Gene Symbol
COX5B
Additional COX5b Products
Product Documents for COX5b Antibody - BSA Free
Product Specific Notices for COX5b Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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