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CPI17 alpha Antibody (3A7)

Novus Biologicals, part of Bio-Techne | Catalog # H00094274-M01

Novus Biologicals, part of Bio-Techne
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H00094274-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PPP1R14A (AAH21089, 1 a.a. ~ 147 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP

Reactivity Notes

Human. Other species not tested.

Specificity

PPP1R14A - protein phosphatase 1, regulatory (inhibitor) subunit 14A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CPI17 alpha Antibody (3A7)

Western Blot: CPI17 alpha Antibody (3A7) [H00094274-M01]

Western Blot: CPI17 alpha Antibody (3A7) [H00094274-M01]

Western Blot: CPI17 alpha Antibody (3A7) [H00094274-M01] - Analysis of PPP1R14A expression in transfected 293T cell line by PPP1R14A monoclonal antibody (M01), clone 3A7.Lane 1: PPP1R14A transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CPI17 alpha Antibody (3A7) [H00094274-M01] - Detection limit for recombinant GST tagged PPP1R14A is approximately 0.1ng/ml as a capture antibody.

Applications for CPI17 alpha Antibody (3A7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CPI17 alpha

PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM]

Alternate Names

17 kDa PKC-potentiated inhibitory protein of PP1, CPI-1717-kDa PKC-potentiated inhibitory protein of PP1, CPI1717-KDa protein, PKC-potentiated inhibitory protein of PP1, PPP1INL, Protein kinase C-potentiated inhibitor protein of 17 kDa, protein phosphatase 1 regulatory subunit 14A, protein phosphatase 1, regulatory (inhibitor) subunit 14A

Entrez Gene IDs

94274 (Human)

Gene Symbol

PPP1R14A

OMIM

608153 (Human)

Additional CPI17 alpha Products

Product Documents for CPI17 alpha Antibody (3A7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CPI17 alpha Antibody (3A7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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