Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H10

Format

BSA Free

Concentration

1.0 mg/ml

View all CPI17 alpha Antibodies »

Product Specifications

Immunogen

Recombinant human CPI17 alpha (1-147aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CPI17 alpha Antibody (4H10) - BSA Free

Western Blot: CPI17 alpha Antibody (4H10)BSA Free [NBP1-04336]

Western Blot: CPI17 alpha Antibody (4H10)BSA Free [NBP1-04336]

Western Blot: CPI17 alpha Antibody (4H10) [NBP1-04336] - The Cell lysates of A549 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western Blot: CPI17 alpha Antibody (4H10) [NBP1-04336] -

Western Blot: CPI17 alpha Antibody (4H10) [NBP1-04336] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : A549 cell lysate Lane 2. : NIH-3T3 cell lysate

Applications for CPI17 alpha Antibody (4H10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:250-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CPI17 alpha

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.

Alternate Names

17 kDa PKC-potentiated inhibitory protein of PP1, CPI-1717-kDa PKC-potentiated inhibitory protein of PP1, CPI1717-KDa protein, PKC-potentiated inhibitory protein of PP1, PPP1INL, Protein kinase C-potentiated inhibitor protein of 17 kDa, protein phosphatase 1 regulatory subunit 14A, protein phosphatase 1, regulatory (inhibitor) subunit 14A

Gene Symbol

PPP1R14A

UniProt

NP_150281 (Human)

Additional CPI17 alpha Products

Product Documents for CPI17 alpha Antibody (4H10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CPI17 alpha Antibody (4H10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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