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CPNE5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84406

Novus Biologicals, part of Bio-Techne
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NBP1-84406
NBP1-84406-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MEQPEDMASLSEFDSLAGSIPATKVEITVSCRNLLDKDMFSKSDPLCVMYTQGMENKQW

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:31284824).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CPNE5 Antibody

Immunohistochemistry-Paraffin: CPNE5 Antibody [NBP1-84406]

Immunohistochemistry-Paraffin: CPNE5 Antibody [NBP1-84406]

Immunohistochemistry-Paraffin: CPNE5 Antibody [NBP1-84406] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP1-84406 antibody. Corresponding CPNE5 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: CPNE5 Antibody [NBP1-84406]

Immunocytochemistry/ Immunofluorescence: CPNE5 Antibody [NBP1-84406]

Immunocytochemistry/Immunofluorescence: CPNE5 Antibody [NBP1-84406] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CPNE5 Antibody [NBP1-84406]

Immunohistochemistry-Paraffin: CPNE5 Antibody [NBP1-84406]

Immunohistochemistry-Paraffin: CPNE5 Antibody [NBP1-84406] - Staining of human skeletal muscle shows no granular cytoplasmic positivity in myocytes as expected.

Applications for CPNE5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CPNE5

Copine 5 or Copine V (CPNE5) is a member of the copine family. The copines are ubiquitous, Ca2+ dependent, phospholipid-binding proteins. Due to the copine's phospholipid binding capabilities, it is thought that they play a role in membrane trafficking. CPNE5 contains two N-terminal type II C2 domains and, in the C-terminus, an integrin A domain-like sequence. Recent studies with Arabidopsis suggest that copines may also play a role in cell division and growth.

Alternate Names

copine VKIAA1599CPN5COPN5, copine-5, DKFZp666C234

Gene Symbol

CPNE5

Additional CPNE5 Products

Product Documents for CPNE5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CPNE5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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