Skip to main content

CPS1 Antibody (SPM615) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47905

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM615

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant human CPS1 protein (Uniprot: P31327)

Localization

Finely granular cytoplasmic

Marker

Hepatocellular Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44552).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CPS1 Antibody (SPM615) - Azide and BSA Free

Immunohistochemistry-Paraffin: CPS1 Antibody (SPM615) - Azide and BSA Free [NBP2-47905]

Immunohistochemistry-Paraffin: CPS1 Antibody (SPM615) - Azide and BSA Free [NBP2-47905]

Immunohistochemistry-Paraffin: CPS1 Antibody (SPM615) - Azide and BSA Free [NBP2-47905] - Human Hepatocellular Carcinoma stained with CPS1 Monoclonal Antibody (SPM615).

Applications for CPS1 Antibody (SPM615) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CPS1

The protein encoded by the CPS1 gene is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. There are two isozymes of this enzyme, and the encoded protein is the mitochondrial form. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion.

Alternate Names

carbamoyl-phosphate synthase [ammonia], mitochondrial, carbamoyl-phosphate synthase 1, mitochondrial, carbamoyl-phosphate synthetase 1, mitochondrial, carbamoylphosphate synthetase I, Carbamoyl-phosphate synthetase I, CPSase I, CPSASE1, EC 6.3.4.16

Gene Symbol

CPS1

Additional CPS1 Products

Product Documents for CPS1 Antibody (SPM615) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CPS1 Antibody (SPM615) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...