CPSF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79826

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CPSF4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 220 and 245 of human cleavage and polyadenylation specific factor 4, 30kD subunit using the numbering given in entry NP_006684.1 (GeneID 10898).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CPSF4 Antibody

Western Blot: CPSF4 Antibody [NB100-79826]

Western Blot: CPSF4 Antibody [NB100-79826]

Western Blot: CPSF4 Antibody [NB100-79826] - Detection of Human and Mouse CPSF30 on HeLa whole cell lysate using NB100-79826. CPSF30 was also immunoprecipitated by rabbit anti-CPSF30 antibody NB10-79827.

Applications for CPSF4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CPSF4

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.

Alternate Names

30kD, cleavage and polyadenylation specific factor 4, 30kD subunit, cleavage and polyadenylation specific factor 4, 30kDa, CPSF 30 kDa subunit, neb-1, No arches homolog, no arches-like zinc finger protein, NS1 effector domain-binding protein 1

Entrez Gene IDs

10898 (Human); 54188 (Mouse)

Gene Symbol

CPSF4

UniProt

O95639 (Human)

Additional CPSF4 Products

Product Documents for CPSF4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CPSF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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