CPSF6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99189
![Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]](https://resources.bio-techne.com/images/products/CPSF6-Antibody-Immunohistochemistry-Paraffin-NBP2-99189-img0004.jpg "Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]")
![Immunoprecipitation: CPSF6 Antibody [NBP2-99189]](https://resources.bio-techne.com/images/products/CPSF6-Antibody-Immunoprecipitation-NBP2-99189-img0001.jpg "Immunoprecipitation: CPSF6 Antibody [NBP2-99189]")
![Western Blot: CPSF6 Antibody [NBP2-99189]](https://resources.bio-techne.com/images/products/CPSF6-Antibody-Western-Blot-NBP2-99189-img0003.jpg "Western Blot: CPSF6 Antibody [NBP2-99189]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CPSF6 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CPSF6.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CPSF6 Antibody
Western Blot: CPSF6 Antibody [NBP2-99189]
Western Blot: CPSF6 Antibody [NBP2-99189] - Anti-CPSF6 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa (We are unsure as to the identity of these extra bands).![Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody [NBP2-99189]](https://resources.bio-techne.com/images/products/CPSF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99189-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody [NBP2-99189]")
Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody [NBP2-99189]
Immunocytochemistry/Immunofluorescence: CPSF6 Antibody [NBP2-99189] - Immunofluorescence staining of CPSF6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CPSF6 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.![Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]](https://resources.bio-techne.com/images/products/CPSF6-Antibody-Immunohistochemistry-Paraffin-NBP2-99189-img0005.jpg "Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]")
Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189]
Immunohistochemistry-Paraffin: CPSF6 Antibody [NBP2-99189] - Immunochemical staining of human CPSF6 in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).Applications for CPSF6 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CPSF6
Alternate Names
CFIM, CFIm68, CFIM68CPSF 68 kDa subunit, CFIMcleavage and polyadenylation specific factor 6, 68kD subunit, cleavage and polyadenylation specific factor 6, 68kDa, Cleavage and polyadenylation specificity factor 68 kDa subunit, cleavage and polyadenylation specificity factor subunit 6, HPBRII-4, HPBRII-7, pre-mRNA cleavage factor I, 68kD subunit, pre-mRNA cleavage factor Im (68kD), Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7
Gene Symbol
CPSF6
Additional CPSF6 Products
Product Documents for CPSF6 Antibody
Product Specific Notices for CPSF6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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