CPT1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59608

Novus Biologicals, part of Bio-Techne
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NBP1-59608-100ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all CPT1A Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to CPT1A(carnitine palmitoyltransferase 1A (liver)) The peptide sequence was selected from the middle region of CPT1A (NP_001027017). Peptide sequence LSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLIN. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for CPT1A Antibody

Western Blot: CPT1A Antibody [NBP1-59608]

Western Blot: CPT1A Antibody [NBP1-59608]

Western Blot: CPT1A Antibody [NBP1-59608] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CPT1A.
Western Blot: CPT1A Antibody [NBP1-59608]

Western Blot: CPT1A Antibody [NBP1-59608]

Western Blot: CPT1A Antibody [NBP1-59608] - 293T cells lysate, concentration 0.2-1 ug/ml.
Western Blot: CPT1A Antibody [NBP1-59608]

Western Blot: CPT1A Antibody [NBP1-59608]

Western Blot: CPT1A Antibody [NBP1-59608] - Sample Tissue: 293T, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12

Applications for CPT1A Antibody

Application
Recommended Usage

Immunohistochemistry

1:300

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CPT1A

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Long Name

Carnitine O-palmitoyltransferase 1, liver isoform

Alternate Names

CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21

Entrez Gene IDs

1374 (Human)

Gene Symbol

CPT1A

UniProt

P50416 (Human)

Additional CPT1A Products

Product Documents for CPT1A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CPT1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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