Key Product Details

Species Reactivity

Validated:

Mouse, Cre/lox System

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for Cre Antibody [DyLight 405]

Immunogen

A synthetic peptide made to a portion of bacteriophage protein CRE (within amino acids 50-100). [UniProt# P06956]

Reactivity Notes

Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transfected human cells (HeLa) and Cre-transgenic mice. Mouse reactivity reported in scientific literature (PMID: 28194015).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Cre Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cre

Cre recombinase or cyclization recombination enzyme is a bacteriophage-P1 enzyme belonging to large family of recombinases which are required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state. Cre is well known for facilitating a site-specific recombination between two 34 base pair loxP sites. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule, and the excised DNA segment is subsequently degraded. Cre and lox comprises Cre/lox system which has been used extensively for multiple in vitro and in vivo applications including targeted gene deletions, gene-specific humanized animal models, as well as in plants and microorganisms stocks.

Alternate Names

Cyclization recombinase

Additional Cre Products

Product Documents for Cre Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cre Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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