Cre Antibody

Western Blot: Cre Antibody [NB100-56134]

Western Blot: Cre Antibody - Unpurified [NB100-56134] - Lane 1: wild-type mouse (negative control). Lanes 2 and 3: brain from 2 types of Cre transgenic mice. Lane 4: recombinant Cre (positive control). Full-length Cre is detected at ~36-38 kDa. The lower molecular weight bands in lane 4 are breakdown products from the recombinant Cre protein.

Immunohistochemistry: Cre Antibody [NB100-56134]

Immunohistochemistry: Cre Antibody - Unpurified [NB100-56134] - Cre Antibody [NB100-56134] - Analysis of Cre in a FFPE tissue section from the hippocampus of a Cre transgenic mouse at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.

Western Blot: Cre Antibody [NB100-56134] -

Western Blot: Cre Antibody [NB100-56134] - Generation, identification, & Rpe65 expression analysis of Rpe65CreERT2 mice.(A) KI strategy to introduce a P2A-CreERT2 coding sequence in-frame with the final coding exon (exon 14) of the Rpe65 gene. Primer-binding sites & expected PCR product sizes are shown below the wild-type & targeted alleles. The neomycin cassette in the targeting vector was removed during expansion of the embryonic stem cell clone. FLP-recombinase was subsequently bred out by crossing with C57BL/6 mice. The KI allele allows cotranslational expression of RPE65-P2A & CreERT2 as 2 separate polypeptides. The modified RPE65 protein contains an additional G534SGATNFSLLKQAGDVEENPG554 polypeptide sequence at its C-terminus, while CreERT2 contains an additional Pro residue at its N-terminus. (B) Genotyping results from wild-type, Cre+/–, & Cre+/+ animals. (C) Western blot analysis of RPE65 expression. After normalization to the alpha-tubulin loading controls, the RPE65 expression levels in Cre+/– & Cre+/+ mice were estimated to be 59.7% & 1.1% of that for wild-type mice. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33784255), licensed under a CC-BY license. Not internally tested by Novus Biologicals.