Creatine kinase MT 1B Antibody (2C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00001159-M04

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Creatine kinase MT 1B Antibodies »

Product Specifications

Immunogen

CKMT1B (NP_066270, 327 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVHIKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGGVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPTPVIHTKH

Specificity

CKMT1B - creatine kinase, mitochondrial 1B (2C8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Creatine kinase MT 1B Antibody (2C8)

Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04]

Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04]

Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04] - Analysis of CKMT1B expression in transfected 293T cell line by CKMT1B monoclonal antibody (M04), clone 2C8. Lane 1: CKMT1B transfected lysatE (47 KDa). Lane 2: Non-transfected lysate.
Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04]

Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04]

Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04] - Western blot analysis of CKMT1B over-expressed 293 cell line, cotransfected with CKMT1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed H00001159-M04. GAPDH (36.1 kDa) used as loading control.
Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04]

Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04]

Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04] - Analysis of CKMT1B expression in A-431 (Cat # L015V1).
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Applications for Creatine kinase MT 1B Antibody (2C8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Creatine kinase MT 1B

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]

Alternate Names

Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1B, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1B, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, UMTCK, U-MtCK

Entrez Gene IDs

1159 (Human)

Gene Symbol

CKMT1B

UniProt

NP_066270 (Human)

Additional Creatine kinase MT 1B Products

Product Documents for Creatine kinase MT 1B Antibody (2C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Creatine kinase MT 1B Antibody (2C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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