CREB [p Ser133] Antibody (M415)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23244
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M415
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CREB [p Ser133] Antibody (M415)
Immunogen
Clone M415 was generated from phospho-CREB (Ser-133) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-133 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB. The site is also conserved in CREM (Ser-136) and ATF1 (Ser-63). [UniProt# P16220]
Modification
p Ser133
Specificity
The antibody detects a 43 kDa protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, as well as A431 treated with EGF and PC12 treated with NGF or Forskolin. This reactivity is not observed after lambda phosphatase treatment.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for CREB [p Ser133] Antibody (M415)
![CREB [p Ser133] Antibody (M415)](https://resources.bio-techne.com/images/products/nbp3-23244_mouse-creb-p-ser133-mab-m415-19920231915415.jpg "Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] -")
Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] -
Immunocytochemistry/Immunofluorescence: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Immunocytochemical labeling of phosphorylated CREB in calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with mouse monoclonal CREB (Ser-133). The antibody was detected using goat anti-mouse DyLight(R) 594.![CREB [p Ser133] Antibody (M415)](https://resources.bio-techne.com/images/products/nbp3-23244_mouse-creb-p-ser133-mab-m415-199202312523628.jpg "Western Blot: CREB [p Ser133] Antibody (M415) [NBP3-23244] -")
Western Blot: CREB [p Ser133] Antibody (M415) [NBP3-23244] -
Western Blot: CREB [p Ser133] Antibody (M415) [NBP3-23244] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3 & 5) then the blots were treated with lambda phosphatase (lanes 2, 4 & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).Applications for CREB [p Ser133] Antibody (M415)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: CREB
Long Name
cAMP Response Element-binding Protein
Alternate Names
CREB1
Gene Symbol
CREB1
Additional CREB Products
Product Documents for CREB [p Ser133] Antibody (M415)
Product Specific Notices for CREB [p Ser133] Antibody (M415)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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