CREB [p Ser271] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23250
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CREB [p Ser271] Antibody
Immunogen
Phospho-CREB (Ser-271) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-271 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB. The site has some homology to the conserved site in ATF1 (Ser-198) and CREM (Ser-287). [UniProt# P16220]
Modification
p Ser271
Specificity
The antibody detects a 43 kDa protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human A431, Jurkat, and rat PC12 cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for CREB [p Ser271] Antibody
![CREB [p Ser271] Antibody](https://resources.bio-techne.com/images/products/nbp3-23250_rabbit-creb-p-ser271-pab-1992023199286.jpg "Immunocytochemistry/Immunofluorescence: CREB [p Ser271] Antibody [NBP3-23250] -")
Immunocytochemistry/Immunofluorescence: CREB [p Ser271] Antibody [NBP3-23250] -
Immunocytochemistry/Immunofluorescence: CREB [p Ser271] Antibody [NBP3-23250] - Immunocytochemical labeling of phosphorylated CREB in control and calyculin A-treated A431 cells. The cells were fixed in paraformaldehyde and permeabilized using NP-40 before labeling with rabbit polyclonal CREB (Ser-271). The antibody was detected using goat anti-rabbit DyLight(R) 594.![CREB [p Ser271] Antibody](https://resources.bio-techne.com/images/products/nbp3-23250_rabbit-creb-p-ser271-pab-189202317355213.jpg "Western Blot: CREB [p Ser271] Antibody [NBP3-23250] -")
Western Blot: CREB [p Ser271] Antibody [NBP3-23250] -
Western Blot: CREB [p Ser271] Antibody [NBP3-23250] - Western blot analysis of human A431 cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-CREB (N-terminal region) (lanes 1 & 2), anti-CREB (Ser-133) (lanes 3 & 4), and anti-CREB (Ser-271) (lanes 5 & 6).Applications for CREB [p Ser271] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: CREB
Long Name
cAMP Response Element-binding Protein
Alternate Names
CREB1
Gene Symbol
CREB1
Additional CREB Products
Product Documents for CREB [p Ser271] Antibody
Product Specific Notices for CREB [p Ser271] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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