CRELD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88917

Novus Biologicals, part of Bio-Techne
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NBP1-88917
NBP1-88917-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRELD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GAFPILTDLTPETTRRWKLGSHPHSTYVKMKMQRDEATFPGLYGKQVAKLGSQSRQSDRGTRL

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRELD1 Antibody

Western Blot: CRELD1 Antibody [NBP1-88917]

Western Blot: CRELD1 Antibody [NBP1-88917]

Western Blot: CRELD1 Antibody [NBP1-88917] - Analysis in control (vector only transfected HEK293T lysate) and CRELD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CRELD1 Antibody [NBP1-88917]

Immunocytochemistry/ Immunofluorescence: CRELD1 Antibody [NBP1-88917]

Immunocytochemistry/Immunofluorescence: CRELD1 Antibody [NBP1-88917] - Staining of human cell line U-251 MG shows localization to nucleoli & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CRELD1 Antibody [NBP1-88917]

Immunohistochemistry-Paraffin: CRELD1 Antibody [NBP1-88917]

Immunohistochemistry-Paraffin: CRELD1 Antibody [NBP1-88917] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for CRELD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRELD1

Epidermal growth factor (EGF; MIM 131530)-like repeats are a class of cysteine-rich domains that mediate interactions between proteins of diverse function. EGF domains are found in proteins that are either completely secreted or have transmembrane regions that tether the protein to the cell surface. CRELD1 is the founding member of a family of matricellular proteins.[supplied by OMIM]

Long Name

Cysteine-rich Protein With EGF-like Domains 1

Alternate Names

AVSD2, CIRRIN

Gene Symbol

CRELD1

Additional CRELD1 Products

Product Documents for CRELD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CRELD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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