CRISP-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52012
Key Product Details
Species Reactivity
Human, Porcine, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QHSDPEDRKTSTR corresponding to internal region according to NP_003287.1.
Epitope
C-QHSDPEDRKTSTR
Reactivity Notes
Expected from sequence similarity: Porcine, Canine
Specificity
Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for CRISP-2 Antibody
![Western Blot: CRISP-2 Antibody [NBP1-52012]](https://resources.bio-techne.com/images/products/CRISP-2-Antibody-Western-Blot-NBP1-52012-img0003.jpg "Western Blot: CRISP-2 Antibody [NBP1-52012]")
Western Blot: CRISP-2 Antibody [NBP1-52012]
Western Blot: CRISP-2 Antibody [NBP1-52012] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B.![Immunohistochemistry-Paraffin: CRISP-2 Antibody [NBP1-52012]](https://resources.bio-techne.com/images/products/CRISP-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52012-img0004.jpg "Immunohistochemistry-Paraffin: CRISP-2 Antibody [NBP1-52012]")
Immunohistochemistry-Paraffin: CRISP-2 Antibody [NBP1-52012]
Immunohistochemistry-Paraffin: CRISP-2 Antibody [NBP1-52012] - 3.75ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for CRISP-2 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
1:1000
Application Notes
Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Primary incubations were for 1 hour.
Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Testis.
IHC: Paraffin embedded Human Testis.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CRISP-2
Long Name
Cysteine-rich Secretory Protein 2
Alternate Names
CRISP2, GAPDL5, TPX1, TSP1
Entrez Gene IDs
7180 (Human)
Gene Symbol
CRISP2
UniProt
Additional CRISP-2 Products
Product Documents for CRISP-2 Antibody
Product Specific Notices for CRISP-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...