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Crk Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32044

Novus Biologicals, part of Bio-Techne
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NBP1-32044

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.

Localization

Cytoplasm , Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Crk Antibody

Western Blot: Crk Antibody [NBP1-32044]

Western Blot: Crk Antibody [NBP1-32044]

Western Blot: Crk Antibody [NBP1-32044] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with CRK antibody [N2C3].
Immunocytochemistry/ Immunofluorescence: Crk Antibody [NBP1-32044]

Immunocytochemistry/ Immunofluorescence: Crk Antibody [NBP1-32044]

Immunocytochemistry/Immunofluorescence: Crk Antibody [NBP1-32044] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Western Blot: Crk Antibody [NBP1-32044]

Western Blot: Crk Antibody [NBP1-32044]

Western Blot: Crk Antibody [NBP1-32044] - Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.

Applications for Crk Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 40% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Crk

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog

Alternate Names

adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)

Gene Symbol

CRK

Additional Crk Products

Product Documents for Crk Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Crk Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.