CrkL [p Tyr207] Antibody (JE55-58)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32757
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE55-58
Concentration
1 mg/ml
Product Summary for CrkL [p Tyr207] Antibody (JE55-58)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr207 of human CrkL protein. (Uniprot: P46109)
Modification
p Tyr 207
Localization
cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm, protein-containing complex.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CrkL [p Tyr207] Antibody (JE55-58)
![CrkL [p Tyr207] Antibody (JE55-58)](https://resources.bio-techne.com/images/products/nbp3-32757_rabbit-crkl-p-tyr207-mab-je55-58-288202412461638.jpg "Western Blot: CrkL [p Tyr207] Antibody (JE55-58) [NBP3-32757]")
Western Blot: CrkL [p Tyr207] Antibody (JE55-58) [NBP3-32757]
Western Blot: CrkL [p Tyr207] Antibody (JE55-58) [NBP3-32757] - Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (NBP3-32757) at 1/2,000 dilution.Lane 1: K-562 cell lysate
Lane 2: K-562 treated pp for 1 hour cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 34 kDa
Observed band size: 38 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32757) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for CrkL [p Tyr207] Antibody (JE55-58)
Application
Recommended Usage
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CrkL
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein
Alternate Names
crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Gene Symbol
CRKL
Additional CrkL Products
Product Documents for CrkL [p Tyr207] Antibody (JE55-58)
Product Specific Notices for CrkL [p Tyr207] Antibody (JE55-58)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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