CrkL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55130

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-55130
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CrkL Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CrkL Antibody

Western Blot: CrkL Antibody [NBP2-55130]

Western Blot: CrkL Antibody [NBP2-55130]

Western Blot: CrkL Antibody [NBP2-55130] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: CrkL Antibody [NBP2-55130]

Immunocytochemistry/ Immunofluorescence: CrkL Antibody [NBP2-55130]

Immunocytochemistry/Immunofluorescence: CrkL Antibody [NBP2-55130] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Applications for CrkL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CrkL

CrkL is a widely-expressed 39 kD adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoetic origin. Through its SH2 and SH3 domains CrkL initiates interactions with numerous downstream proteins such as CBL, Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. It is involved in processes such as adhesion, migration and immune responses. Furthermore, CrkL plays an essential role in Chronic Myelogenous Leukemia and is constitutively phosphorylated by the Bcr-Abl kinase in this disease.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Alternate Names

crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Gene Symbol

CRKL

Additional CrkL Products

Product Documents for CrkL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CrkL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.