CrkL [p Tyr207] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23257
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Xenopus
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CrkL [p Tyr207] Antibody
Immunogen
Phospho-Crk (Tyr-207) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-207 in human CrkL. This peptide sequence is well conserved in mouse and rat CrkL, and has 40% homology with the conserved site in Crk II (Tyr-221). The site is not found in Crk I. [UniProt# P46109]
Modification
p Tyr207
Specificity
The antibody detects a 38 kDa protein corresponding to the molecular mass of CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for CrkL [p Tyr207] Antibody
![CrkL [p Tyr207] Antibody](https://resources.bio-techne.com/images/products/nbp3-23257_rabbit-crkl-p-tyr207-pab-199202312403715.jpg "Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] -")
Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] -
Western Blot: CrkL [p Tyr207] Antibody [NBP3-23257] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate CrkL (lanes 2 & 4), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2) and CrkL (Tyr-207) phospho-specific NBP3-23257 (lanes 3 & 4).Applications for CrkL [p Tyr207] Antibody
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: CrkL
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein
Alternate Names
crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Gene Symbol
CRKL
Additional CrkL Products
Product Documents for CrkL [p Tyr207] Antibody
Product Specific Notices for CrkL [p Tyr207] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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