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CRLF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84382

Novus Biologicals, part of Bio-Techne
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NBP1-84382
NBP1-84382-25ul

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IEHGVNTAEDLVREGEIAMLGGVGEENEKLWSFTKKASHIQLDSLPEVPLLVDVPCLSAQLDDSILNIVKDHIFKHGTVASRPPVQIEELIEKPGGIIVRWCKVDDDFTAQDYRLQFRKCTSNHFEDVYVGSE

Reactivity Notes

Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRLF3 Antibody

Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382]

Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382]

Immunohistochemistry-Paraffin: CRLF3 Antibody [NBP1-84382] - Staining in human lymph node and pancreas tissues using anti-CRLF3 antibody. Corresponding CRLF3 RNA-seq data are presented for the same tissues.
Western Blot: CRLF3 Antibody [NBP1-84382]

Western Blot: CRLF3 Antibody [NBP1-84382]

Western Blot: CRLF3 Antibody [NBP1-84382] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CRLF3 Antibody [NBP1-84382]

Immunocytochemistry/ Immunofluorescence: CRLF3 Antibody [NBP1-84382]

Immunocytochemistry/Immunofluorescence: CRLF3 Antibody [NBP1-84382] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Applications for CRLF3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRLF3

Alternate Names

CREME-9, CREME9MGC20661, cytokine receptor-like factor 3, Cytokine receptor-like molecule 9, Cytokine receptor-related protein 4, CYTOR4cytokine receptor related protein 4, FRWS, P48, p48.2, type I cytokine receptor like factor, Type I cytokine receptor-like factor p48

Gene Symbol

CRLF3

Additional CRLF3 Products

Product Documents for CRLF3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CRLF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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