CRMP2 [p Thr555] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23267

Novus Biologicals, part of Bio-Techne
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NBP3-23267
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CRMP2 Antibodies »

Product Specifications

Immunogen

Phospho-CRMP2 (Thr-555) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Thr-555 in human CRMP2. This sequence is conserved in rat and mouse CRMP2 and the phospho-site is not conserved in other CRMP family members. [UniProt# Q16555]

Modification

p Thr555

Specificity

This antibody was cross-adsorbed to unphosphorylated CRMP2 (Thr-555) before affinity purification using phospho-CRMP2 (Thr-555) peptide (without carrier). The antibody detects a 70 kDa protein corresponding to CRMP2 on immunoblots of rat PC12 or mouse C2C12 cells treated with calyculin A. This reactivity can be specifically blocked using phospho-CRMP2 (Thr-555) peptide (CX2255).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for CRMP2 [p Thr555] Antibody

CRMP2 [p Thr555] Antibody

Western Blot: CRMP2 [p Thr555] Antibody [NBP3-23267] -

Western Blot: CRMP2 [p Thr555] Antibody [NBP3-23267] - Western blot of rat PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) antibody (lanes 1 & 2) or anti-CRMP2 (Thr-555) antibody (lanes 3 & 4).
CRMP2 [p Thr555] Antibody

Immunocytochemistry/Immunofluorescence: CRMP2 [p Thr555] Antibody [NBP3-23267] -

Immunocytochemistry/Immunofluorescence: CRMP2 [p Thr555] Antibody [NBP3-23267] - Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.

Applications for CRMP2 [p Thr555] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CRMP2

Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease.

Alternate Names

Collapsin response mediator protein 2, CRMP-2, CRMP2N2A3, DHPRP2, dihydropyrimidinase-like 2, dihydropyrimidinase-like 2 long form, dihydropyrimidinase-related protein 2, DRP2collapsin response mediator protein hCRMP-2, DRP-2ULIP-2, ULIP2, Unc-33-like phosphoprotein 2

Gene Symbol

DPYSL2

Additional CRMP2 Products

Product Documents for CRMP2 [p Thr555] Antibody

Product Datasheets

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Product Specific Notices for CRMP2 [p Thr555] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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