CROT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31441

Novus Biologicals, part of Bio-Techne
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NBP1-31441
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CROT Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CROT. The exact sequence is proprietary.

Localization

Peroxisome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CROT Antibody

Western Blot: CROT Antibody [NBP1-31441]

Western Blot: CROT Antibody [NBP1-31441]

Western Blot: CROT Antibody [NBP1-31441] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE COT antibody, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: CROT Antibody [NBP1-31441]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-31441]

Immunohistochemistry-Paraffin: CROT Antibody [NBP1-31441] - Paraffin-embedded Ca922 xenograft, using antibody at 1:100 dilution.
Western Blot: CROT Antibody [NBP1-31441]

Western Blot: CROT Antibody [NBP1-31441]

Western Blot: CROT Antibody [NBP1-31441] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.

Applications for CROT Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CROT

Carnitine octanoyltransferase (EC 2.3.1.137) is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria. See also CRAT (MIM 600184). Van der Leij et al. (2000) [PubMed 11001805] reviewed the function, structural features, and phylogenetics of human carnitine acyltransferase genes, including CROT.[supplied by OMIM]

Alternate Names

carnitine O-octanoyltransferase, COTperoxisomal carnitine O-octanoyltransferase, EC 2.3.1, EC 2.3.1.137, peroxisomal carnitine acyltransferase, peroxisomal carnitine octanoyltransferase

Entrez Gene IDs

54677 (Human); 74114 (Mouse)

Gene Symbol

CROT

UniProt

Q9UKG9 (Human)

Additional CROT Products

Product Documents for CROT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CROT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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