CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Application

Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CRTH-2

GPR44, also called CRTH2, is a Prostanoid Receptor that binds prostaglandin D2. PGD2 signals through CRTH2 to induce Th2, eosinophil, and basophil chemotaxis in a G-alpha(i) dependent manner. CRTH2 may play a role in mast cell-mediated immune responses such as those involved in allergic reactions. This receptor is upregulated in activated T helper-2 (Th2) cells, peripheral blood basophils, and eosinophils, but not in Th1 lineage cells. It may be a useful marker for Th2 and Tc2 cells. CRTH2 has been reported to be expressed in the central nervous system, peripheral tissues, and in blood cells, particularly activated T helper-2 cells. Two transcripts have been isolated, a 3.5-kb transcript expressed primarily in the thalamus, frontal cortex, pons, and hippocampus, and a 3.4-kb transcript expressed in fetal liver, leukocytes, and thymus. ESTs have been isolated from B-cell/lung/testis, brain, kidney, liver/spleen, placenta, prostate, testis, and thymus libraries.

Long Name

Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells

Alternate Names

CD294, CRTH2, GPR44, PGD2R, PTGDR2

Gene Symbol

PTGDR2

Additional CRTH-2 Products

Product Documents for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93564CL1

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all CRTH-2 Antibodies »

Product Summary for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Description

Applications for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CRTH-2

Long Name

Alternate Names

Gene Symbol

Additional CRTH-2 Products

Product Documents for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CRTH-2/GPR44 Antibody [CoraFluor™ 1]

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