CRX/CORD2 Antibody (4G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00001406-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Fish

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Chemotaxis, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRX Antibodies »

Product Specifications

Immunogen

CRX (NP_000545, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMAYMNPGPHYSVNALALSGPSVDLMHQAVPYPSAPRKQRRERTTFTRSQLEELEALFAKTQYPDVYAREEVALKINLPESRVQVWFKNRRAKCR

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25392503). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

CRX - cone-rod homeobox

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CRX/CORD2 Antibody (4G11)

Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02]

Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02]

Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M02), clone 4G11.Lane 1: CRX transfected lysate(32 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CRX/CORD2 Antibody (4G11) [H00001406-M02]

Immunocytochemistry/ Immunofluorescence: CRX/CORD2 Antibody (4G11) [H00001406-M02]

Immunocytochemistry/Immunofluorescence: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Retinal development is recapitulated in differentiating stem cells. Immunostaining of day 49 cultures shows remnants of retinal organization. CRX+ photoreceptor progenitors in green and mCherry+ RGCs in magenta. CRX+ cells - white arrows - appear to segregate from mCherry+ axons, suggesting division between the outer nuclear layer and the nerve fiber layer. Scale bar?=?500?um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep16595), licensed under a CC-BY license.
Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02]

Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02]

Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02] - CRX monoclonal antibody (M02), clone 4G11 Analysis of CRX expression in IMR-32.
View 1 more images

Applications for CRX/CORD2 Antibody (4G11)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA. ChIP usage reported in scientific literature (PMID 25392503). ICC/IF usage reported in scientific literature (PMID 26563826).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRX

The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.

Long Name

Cone-rod Homeobox Protein

Alternate Names

CORD2, LCA7, OTX3

Entrez Gene IDs

1406 (Human)

Gene Symbol

CRX

UniProt

NP_000545 (Human)

Additional CRX Products

Product Documents for CRX/CORD2 Antibody (4G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRX/CORD2 Antibody (4G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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