CRYBB2 Antibody (1F1)
Novus Biologicals, part of Bio-Techne | Catalog # H00001415-M02
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CRYBB2 (NP_000487.1, 1 a.a. ~ 205 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASDHQTQAGKPQSLNPKIIIFEQENFQGHSHELNGPCPNLKETGVEKAGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPRWDSWTSSRRTDSLSSLRPIKVDSQEHKIILYENPNFTGKKMEIIDDDVPSFHAHGYQEKVSSVRVQSGTWVGYQYPGYRGLQYLLEKGDYKDSSDFGAPHPQVQSVRRIRDMQWHQRGAFHPSN
Specificity
This product is specific for Human CRYBB2 monoclonal antibody (M02), clone 1F1 [Gene ID: 1415].
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for CRYBB2 Antibody (1F1)
![Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]](https://resources.bio-techne.com/images/products/CRYBB2-Antibody-1F1-Western-Blot-H00001415-M02-img0005.jpg "Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]")
Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]
Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - Analysis of CRYBB2 expression in transfected 293T cell line by CRYBB2 monoclonal antibody (M02), clone 1F1.Lane 1: CRYBB2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.![Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]](https://resources.bio-techne.com/images/products/CRYBB2-Antibody-1F1-Western-Blot-H00001415-M02-img0003.jpg "Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]")
Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02]
Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - analysis of CRYBB2 in stably transfected HEK293T cell lysate. Note: membrane was blocked overnight in 3% BSA in TBST at 4C to reduce the background. Image from verified customer review.![ELISA: CRYBB2 Antibody (1F1) [H00001415-M02]](https://resources.bio-techne.com/images/products/CRYBB2-Antibody-1F1-ELISA-H00001415-M02-img0006.jpg "ELISA: CRYBB2 Antibody (1F1) [H00001415-M02]")
ELISA: CRYBB2 Antibody (1F1) [H00001415-M02]
ELISA: CRYBB2 Antibody (1F1) [H00001415-M02] - Detection limit for recombinant GST tagged CRYBB2 is 3 ng/ml as a capture antibody.Applications for CRYBB2 Antibody (1F1)
Application
Recommended Usage
Western Blot
1:100-1:2000
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 31511085).
Reviewed Applications
Read 1 review rated 4 using H00001415-M02 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CRYBB2
Alternate Names
Beta-B2 crystallin, beta-crystallin B2, Beta-crystallin Bp, CCA2, CRYB2A, CRYB2D22S665, crystallin, beta B2, eye lens structural protein
Gene Symbol
CRYBB2
UniProt
Additional CRYBB2 Products
Product Documents for CRYBB2 Antibody (1F1)
Product Specific Notices for CRYBB2 Antibody (1F1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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