CRYBB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13876

Novus Biologicals, part of Bio-Techne
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NBP2-13876
NBP2-13876-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRYBB2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IIDDDVPSFHAHGYQEKVSSVWVRSG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%) Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRYBB2 Antibody

Immunocytochemistry/ Immunofluorescence: CRYBB2 Antibody [NBP2-13876]

Immunocytochemistry/ Immunofluorescence: CRYBB2 Antibody [NBP2-13876]

Immunocytochemistry/Immunofluorescence: CRYBB2 Antibody [NBP2-13876] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876]

Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876]

Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876]

Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876]

Immunohistochemistry-Paraffin: CRYBB2 Antibody [NBP2-13876] - Staining of human eye shows strong positivity in the lens.
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Applications for CRYBB2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

Reported in scientific literature (PMID: 31511085)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRYBB2

Alternate Names

Beta-B2 crystallin, beta-crystallin B2, Beta-crystallin Bp, CCA2, CRYB2A, CRYB2D22S665, crystallin, beta B2, eye lens structural protein

Gene Symbol

CRYBB2

Additional CRYBB2 Products

Product Documents for CRYBB2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CRYBB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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