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CRYBB3 Antibody (4H6)

Novus Biologicals, part of Bio-Techne | Catalog # H00001417-M01

Novus Biologicals, part of Bio-Techne
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H00001417-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CRYBB3 (NP_004067.1, 112 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PHHKLHLFENPAFSGRKMEIVDDDVPSLWAHGFQDRVASVRAINGTWVGYEFPGYRGRQYVFERGEYRHWNEWDASQPQLQSVRRIRDQKWHKRGRFPSS

Specificity

CRYBB3 - crystallin, beta B3 (4H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CRYBB3 Antibody (4H6)

Western Blot: CRYBB3 Antibody (4H6) [H00001417-M01]

Western Blot: CRYBB3 Antibody (4H6) [H00001417-M01]

Western Blot: CRYBB3 Antibody (4H6) [H00001417-M01] - Analysis of CRYBB3 expression in transfected 293T cell line by CRYBB3 monoclonal antibody (M01), clone 4H6.Lane 1: CRYBB3 transfected lysate (Predicted MW: 24.2 KDa).Lane 2: Non-transfected lysate.

Applications for CRYBB3 Antibody (4H6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRYBB3

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, is part of a gene cluster with beta-A4, beta-B1, and beta-B2. [provided by RefSeq]

Alternate Names

Beta-B3 crystallin, beta-crystallin B3, CATCN2, CRYB3MGC125774, crystallin, beta B3, eye lens structural protein, MGC125772, MGC125773

Entrez Gene IDs

1417 (Human)

Gene Symbol

CRYBB3

Additional CRYBB3 Products

Product Documents for CRYBB3 Antibody (4H6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CRYBB3 Antibody (4H6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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