CRYGD Antibody (4E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00001421-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CRYGD Antibodies »

Product Specifications

Immunogen

CRYGD (NP_008822, 75 a.a. ~ 174 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SVRSCRLIPHSGSHRIRLYEREDYRGQMIEFTEDCSCLQDRFRFNEIHSLNVLEGSWVLYELSNYRGRQYLLMPGDYRRYQDWGATNARVGSLRRVIDFS

Specificity

CRYGD (4E12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CRYGD Antibody (4E12)

Western Blot: CRYGD Antibody (4E12) [H00001421-M03]

Western Blot: CRYGD Antibody (4E12) [H00001421-M03]

Western Blot: CRYGD Antibody (4E12) [H00001421-M03] - Analysis of CRYGD expression in HepG2 (Cat # L019V1).
Sandwich ELISA: CRYGD Antibody (4E12) [H00001421-M03] - Detection limit for recombinant GST tagged CRYGD is approximately 0.03ng/ml as a capture antibody.

Applications for CRYGD Antibody (4E12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRYGD

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.

Alternate Names

CACA, CCA3, CCP, CRYG4gamma crystallin 4, cry-g-D, crystallin, gamma D, Gamma-crystallin 4, gamma-crystallin D, Gamma-D-crystallin

Entrez Gene IDs

1421 (Human)

Gene Symbol

CRYGD

OMIM

115700 (Human)

UniProt

NP_008822 (Human)

Additional CRYGD Products

Product Documents for CRYGD Antibody (4E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CRYGD Antibody (4E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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