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CS Citrate Synthase Antibody (CL2579)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36775

Novus Biologicals, part of Bio-Techne
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NBP2-36775
NBP2-36775-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2579

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:ADLIPKEQARIKTFRQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGFSIPECQKLLPKAKGGEEPLPEGLFWLLVTGHIPTEEQVSWL

Epitope

AKGGEEPLPEGLFWL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CS Citrate Synthase Antibody (CL2579)

Western Blot: CS Citrate Synthase Antibody (CL2579) [NBP2-36775]

Western Blot: CS Citrate Synthase Antibody (CL2579) [NBP2-36775]

Western Blot: CS Citrate Synthase Antibody (CL2579) [NBP2-36775] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2579) [NBP2-36775]

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (CL2579) [NBP2-36775]

Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (CL2579) [NBP2-36775] - Staining in HeLa cell line with Anti-CS monoclonal antibody, showing specific mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: CS Citrate Synthase Antibody (CL2579) [NBP2-36775]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2579) [NBP2-36775]

Immunohistochemistry: CS Citrate Synthase Antibody (CL2579) [NBP2-36775] - Staining of human placenta shows granular cytoplasmic positivity in the trophoblast.

Applications for CS Citrate Synthase Antibody (CL2579)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CS Citrate Synthase

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Long Name

CS Citrate Synthase

Alternate Names

citrate (Si)-synthase, citrate synthase, mitochondrial, EC 2.3.3.1

Gene Symbol

CS

UniProt

Additional CS Citrate Synthase Products

Product Documents for CS Citrate Synthase Antibody (CL2579)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CS Citrate Synthase Antibody (CL2579)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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