CSDE1 Antibody

Western Blot: CSDE1 Antibody [NBP1-71915]

Western Blot: CSDE1 Antibody [NBP1-71915] - Purification of Csde1 containing RNP complexes. MEL cells expressing BirA, or expressing BirA plus biotag-Csde1 were lysed, and incubated with streptavidin beads. Supernatant and beads were collected, beads were washed and eluted. Western blot with fractions was stained with anti-Csde1 antibody. Size markers are indicated in kDa. The positions of endogenous and biotagged Csde1 are indicated. A single empty lane has been cropped. The uncropped image is available as Supplementary Figure S5. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-20518-7), licensed under a CC-BY license.

Western Blot: CSDE1 Antibody [NBP1-71915]

Western Blot: CSDE1 Antibody [NBP1-71915] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UNR antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. UNR was also immunoprecipitated by rabbit anti-UNR antibodies NBP1-71913 and NBP1-71914, which recognize upstream epitopes.

Knockdown Validated: CSDE1 Antibody [NBP1-71915]

Knockdown Validated: CSDE1 Antibody [NBP1-71915] - Deletion of the 1st cold shock domain of Csde1 using Crispr/Cas9. Western blot stained for Csde1 and tubulin as loading control. The membrane was cut between Csde1 and tubulin and staining performed on each separately. D1 (2.1) and D2 (2.14) represent out-of-frame deletions (Del), H1 (2.121) and H2 (2.123) in-frame deletions of the 1st cold shock domain (Hm). HET are control clones that harbor 1 deleted (out-of-frame) allele and 1 wt allele. Raw image scans are available as Supplementary Figure S6. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-20518-7), licensed under a CC-BY license.