CTCF Antibody (BLR041F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76420

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR041F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CTCF Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 650 and 700

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CTCF Antibody (BLR041F)

Western Blot: CTCF Antibody (BLR041F) [NBP2-76420]

Western Blot: CTCF Antibody (BLR041F) [NBP2-76420]

Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.
CTCF Antibody (BLR041F)

Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] -

Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF by Simple Western™. Samples: Whole cell lysate (2.0 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.
CTCF Antibody (BLR041F)

Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] -

Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.
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Applications for CTCF Antibody (BLR041F)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100- 1:500

Immunoprecipitation

20 ul/1mg lysate

Simple Western

1:10 - 1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CTCF

CTCF is a versatile 11-Zn-finger transcription factor that may function as a repressor of transcription, activator transcription, or regulator of genomic imprinting. CTCF is a candidate tumor suppressor associated with invasive breast cancer, prostate cancer, and Wilm's tumor.

Long Name

CCCTC-binding Factor

Alternate Names

11-Zinc Finger Protein2, CCCTC-binding Factor

Gene Symbol

CTCF

Additional CTCF Products

Product Documents for CTCF Antibody (BLR041F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CTCF Antibody (BLR041F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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