CTDSPL Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20903
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence QCNVSLKKQRSRS corresponding to internal region according to NP_001008393.1, NP_005799.2.
Epitope
internal region
Specificity
This antibody is expected to recognise both isoforms (NP_001008393.1; NP_005799.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.
Scientific Data Images for CTDSPL Antibody
Immunocytochemistry/Immunofluorescence: CTDSPL Antibody [NBP1-20903] -
Immunocytochemistry/Immunofluorescence: CTDSPL Antibody [NBP1-20903] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: CTDSPL Antibody [NBP1-20903] -
Flow Cytometry: CTDSPL Antibody [NBP1-20903] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for CTDSPL Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:32000
Application Notes
WB: Preliminary testing showed a band at approx 28kDa in nuclear lysate of cell lines A431 and HeLa, after 1-2ug/ml antibody staining (calculated Mwt of 29.9kDa according to NP_005799.2). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CTDSPL
Alternate Names
C3orf8, Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 3, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase-like, CTD small phosphatase-like protein, CTDSP-like, EC 3.1.3, HYA22, NIF1, NIFL, NIF-like protein, NLI-interacting factor 1, Nuclear LIM interactor-interacting factor 1, Protein YA22, PSR1, RB protein serine phosphatase from chromosome 3, RBSP3EC 3.1.3.16, SCP3chromosome 3 open reading frame 8, Small CTD phosphatase 3, Small C-terminal domain phosphatase 3, YA22
Entrez Gene IDs
10217 (Human)
Gene Symbol
CTDSPL
UniProt
Additional CTDSPL Products
Product Documents for CTDSPL Antibody
Product Specific Notices for CTDSPL Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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