CTHRC1 Antibody (166)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89947

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 166

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CTHRC1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CTHRC1 (Accession#: NP_612464.1; Met1-Lys243).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CTHRC1 Antibody (166)

Western Blot: CTHRC1 Antibody (166) [NBP2-89947]

Western Blot: CTHRC1 Antibody (166) [NBP2-89947]

Western Blot: CTHRC1 Antibody (166) [NBP2-89947] - Lane A: HepG2 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:23 kDa
Immunoprecipitation: CTHRC1 Antibody (166) [NBP2-89947]

Immunoprecipitation: CTHRC1 Antibody (166) [NBP2-89947]

Immunoprecipitation: CTHRC1 Antibody (166) [NBP2-89947] - Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-CTHRC1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CTHRC1 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 23 kDa

Applications for CTHRC1 Antibody (166)

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTHRC1

CTHRC1 (Collagen triple helix repeat-containing protein 1) is a 28-30 kDa, secreted glycoprotein that bears similarity to the C1q/TNFalpha-related family of proteins. It is expressed by disparate cell types, including renal epithelium, neurons, osteoblasts, and smooth muscle cells. Functionally, it is recognized to be induced by BMP-2 and to block TGF beta-induced collagen type I and III synthesis. Mouse CTHRC1 is 245 amino acids in length. It contains a 32 amino acid (aa) signal sequence, a 16 aa prosegment (aa 33-48), and a 197 aa mature region that shows one collagen-like domain (aa 59-92). Proteolytic processing may generate multiple CTHRC1 isoforms. There is the potential for an intracellular full-length 33 kDa, 245 aa form, plus extracellular isoforms that are 28 kDa (aa 33-245), 26 kDa (aa 49-245), 20 kDa (aa 98-245) and 18 kDa and 16 kDa in size, the last two representing variants of the 20 kDa form with C-terminal processing. CTHRC1 may undergo dimerization, trimerization and oligomerization. One mouse splice variant shows a deletion of aa 53-126. Full-length mouse CTHRC1 shares 99% and 93% aa sequence identity with rat and human CTHRC1, respectively.

Long Name

Collagen Triple Helix Repeat Containing 1

Alternate Names

NMTC1

Gene Symbol

CTHRC1

Additional CTHRC1 Products

Product Documents for CTHRC1 Antibody (166)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CTHRC1 Antibody (166)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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