Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CTHRC1 Antibodies »

Product Specifications

Immunogen

CTHRC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CTHRC1. The immunogen is located within the first 50 amino acids of CTHRC1. Amino Acid Squence: LPAPSSASEIPKGKQKAQL

Specificity

CTHRC1 antibody is human, mouse and rat reactive. At least two isoforms of CTHRC1 are known to exist; this antibody will detect only the larger isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CTHRC1 Antibody - BSA Free

Western Blot: CTHRC1 AntibodyBSA Free [NBP2-81744]

Western Blot: CTHRC1 AntibodyBSA Free [NBP2-81744]

Western Blot: CTHRC1 Antibody [NBP2-81744] - Analysis of CTHRC1 in rat small intestine tissue lysate with CTHRC1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: CTHRC1 Antibody - BSA Free [NBP2-81744]

Immunocytochemistry/ Immunofluorescence: CTHRC1 Antibody - BSA Free [NBP2-81744]

Immunocytochemistry/Immunofluorescence: CTHRC1 Antibody [NBP2-81744] - Immunofluorescence of CTHRC1 in human small intestine tissue with CTHRC1 antibody at 20 ug/mL.
CTHRC1 Antibody - BSA Free

Immunohistochemistry: CTHRC1 Antibody - BSA Free [NBP2-81744] -

Immunohistochemistry: CTHRC1 Antibody - BSA Free [NBP2-81744] - Immunohistochemistry of CTHRC1 in human small intestine tissue with CTHRC1 antibody at 5 ug/mL.

Applications for CTHRC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTHRC1

CTHRC1 (Collagen triple helix repeat-containing protein 1) is a 28-30 kDa, secreted glycoprotein that bears similarity to the C1q/TNFalpha-related family of proteins. It is expressed by disparate cell types, including renal epithelium, neurons, osteoblasts, and smooth muscle cells. Functionally, it is recognized to be induced by BMP-2 and to block TGF beta-induced collagen type I and III synthesis. Mouse CTHRC1 is 245 amino acids in length. It contains a 32 amino acid (aa) signal sequence, a 16 aa prosegment (aa 33-48), and a 197 aa mature region that shows one collagen-like domain (aa 59-92). Proteolytic processing may generate multiple CTHRC1 isoforms. There is the potential for an intracellular full-length 33 kDa, 245 aa form, plus extracellular isoforms that are 28 kDa (aa 33-245), 26 kDa (aa 49-245), 20 kDa (aa 98-245) and 18 kDa and 16 kDa in size, the last two representing variants of the 20 kDa form with C-terminal processing. CTHRC1 may undergo dimerization, trimerization and oligomerization. One mouse splice variant shows a deletion of aa 53-126. Full-length mouse CTHRC1 shares 99% and 93% aa sequence identity with rat and human CTHRC1, respectively.

Long Name

Collagen Triple Helix Repeat Containing 1

Alternate Names

NMTC1

Gene Symbol

CTHRC1

Additional CTHRC1 Products

Product Documents for CTHRC1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CTHRC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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