Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Gel Supershift Assay, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Gel Supershift Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Ctip1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 775 and the C-terminus (residue 835) of human B-cell CLL/Lymphoma 11A using the numbering given in Swiss-Prot entry Q9H165 (GeneID 53335).

Specificity

NB600-261 is specific for human Bcl-11a protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ctip1 Antibody

Western Blot: Ctip1 Antibody [NB600-261]

Western Blot: Ctip1 Antibody [NB600-261]

Western Blot: Ctip1 Antibody [NB600-261] - Whole cell lysate (50 ug) from L1210, TCMK-1, and 4T1 cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Ctip1 Antibody [NB600-261]

Western Blot: Ctip1 Antibody [NB600-261]

Western Blot: Ctip1 Antibody [NB600-261] - Detection of human Ctip1 (Bcl11a) by western blot. Samples: Whole cell lysate from Ramos (15 and 50 ug), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Ctip1 antibody NB600-261 used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds.
Immunoprecipitation: Ctip1 Antibody [NB600-261]

Immunoprecipitation: Ctip1 Antibody [NB600-261]

Immunoprecipitation: Ctip1 Antibody [NB600-261] - Detection of human Ctip1 (Bcl11a) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells. Antibodies: Affinity purified rabbit anti-Ctip1 antibody NB600-261 used for IP at 6 ul/mg lysate. Ctip1 was also immunoprecipitated by a rabbit anti-Ctip1 antibodies (NB600-258, NB600-259) and rabbit anti-Ctip1 recombinant monoclonal antibody [BLR073G] (NBP2-78710). For blotting immunoprecipitated Ctip1, NBP2-78710 was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.

Applications for Ctip1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Flow Cytometry

1:10-1:1000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
Ctip1 antibody validated for Flow from a verified customer review. Use in Chromatin Immunoprecipitation was reported in scientific publication (PMID: 23741395). Use in Gel suppershift assay reported in scientific literature (PMID:34341563).

Reviewed Applications

Read 1 review rated 4 using NB600-261 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ctip1

The B cell leukemia 11A protein (BCL11A/Evi9/CTIP1) has been implicated in hematopoietic cell development and malignancies. BCL11A is a transcriptional repressor that binds directly to a GC-rich motif and is also recruited to a promoter template via interaction with the orphan nuclear receptor, chicken ovalbumin upstream promoter transcription factor II. In both cases, BCL11A-mediated transcriptional repression is only minimally reversed by trichostatin A, suggesting the possible lack of involvement of class I or II histone deacetylases. Nonetheless, chromatin immunoprecipitation assays revealed that expression of BCL11A in mammalian cells resulted in deacetylation of histones H3 and/or H4 that were associated with the promoter region of a reporter gene. BCL11A mediated transcriptional repression, as well as deacetylation of histone H3/H4 in BCL11A-transfected cells, was partially reversed by nicotinamide, an inhibitor of class III histone deacetylases such as SIRT1. SIRT1 was found to interact directly with BCL11A and was recruited to the promoter template in a BCL11A-dependent manner leading to transcriptional repression. These findings indicate a role for SIRT1 in transcriptional repression mediated by BCL11A in mammalian cells.

Alternate Names

B-cell CLL/lymphoma 11A, B-cell CLL/lymphoma 11A (zinc finger protein), B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2, B-cell lymphoma/leukemia 11A, BCL-11A, BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1, BCL11A-S, BCL11A-XL, C2H2-type zinc finger protein, COUP-TF-interacting protein 1, CTIP1ecotropic viral integration site 9, ecotropic viral integration site 9 homolog, Ecotropic viral integration site 9 protein homolog, EVI-9, EVI9FLJ34997, HBFQTL5, KIAA1809BCL11A-L, Zinc finger protein 856, ZNF856FLJ10173

Entrez Gene IDs

53335 (Human)

Gene Symbol

BCL11A

UniProt

Q9H165 (Human)

Additional Ctip1 Products

Product Documents for Ctip1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ctip1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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