CTR2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05199

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.93 mg/ml

View all CTR2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human CTR2 (within residues 50-100). [Swiss-Prot# O15432]

Localization

Membrane; Multi-pass membrane protein (probable)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CTR2 Antibody - BSA Free

Western Blot: CTR2 AntibodyBSA Free [NBP1-05199]

Western Blot: CTR2 AntibodyBSA Free [NBP1-05199]

Western Blot: CTR2 Antibody [NBP1-05199] - Detection of CTR2 in MEF cell lysate.
Simple Western: CTR2 AntibodyBSA Free [NBP1-05199]

Simple Western: CTR2 AntibodyBSA Free [NBP1-05199]

Simple Western: CTR2 Antibody [NBP1-05199] - Simple Western lane view shows a specific band for CTR2 in 0.5 mg/ml of MEF (left) and Human Placenta (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for CTR2 Antibody - BSA Free

Application
Recommended Usage

Simple Western

10 ug/ml

Western Blot

1 ug/ml
Application Notes
This CTR2 antibody is useful for Western blot, where a band is seen at approx. 71 kDa. The theoretical molecular weight of CTR2 is 15 kDa. The difference between the expected size and the observed size in Western blot is believed to be due to a multimeric form of CTR2 (Bertinato et al).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.93 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTR2

CTR2 (copper transporter 2) belongs to copper transporter (Ctr) (TC 1.A.56) family/SLC31A subfamily and it is a second Cu permease enzyme with high similarity in sequence as well as in overall organization to CTR1 i.e. 3 putative transmembrane domains with an intramembranous MX3M motif, however, it does not have an extended N-terminal domain. CTR2 localizes to intracellular compartments, most notably the endosome or lysosome and it mediates Cu transport into the cytoplasm based on measures of elevated Cu accumulation or metal responsive reporter activation in cells in which CTR2 levels have been altered.

Alternate Names

Copper transporter 2, COPT2SLC13A2, CTR2Solute carrier family 31 member 2, hCTR2probable low affinity copper uptake protein 2, solute carrier family 31 (copper transporters), member 2

Entrez Gene IDs

1318 (Human); 20530 (Mouse)

Gene Symbol

SLC31A2

UniProt

O15432 (Human)

Additional CTR2 Products

Product Documents for CTR2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CTR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.