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CUGBP1/CELF1 Antibody (7A1-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32179

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32179

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 7A1-R

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within Human CUGBP1/CELF1 aa 1-200 / 486. (Uniprot: Q92879)

Localization

Cytoplasm. Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CUGBP1/CELF1 Antibody (7A1-R)

CUGBP1/CELF1 Antibody (7A1-R)

Western Blot: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179]

Western Blot: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Western blot analysis of CUGBP1/CELF1 on different lysates with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: NCI-H226 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: NIH/3T3 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 52 kDa
Observed band size: 55 kDa

Exposure time: 59 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
CUGBP1/CELF1 Antibody (7A1-R)

Immunohistochemistry: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179]

Immunohistochemistry: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32179) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
CUGBP1/CELF1 Antibody (7A1-R)

Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179]

Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Immunocytochemistry analysis of HeLa cells labeling CUGBP1/CELF1 with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.

Applications for CUGBP1/CELF1 Antibody (7A1-R)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CUGBP1/CELF1

Long Name

CUGBP, Elav-Like Family Member 1

Alternate Names

BRUNOL2, CELF1, CUG-BP, CUG-BP1, D2Wsu101e, EDEN-BP, HNAB50, NAB50, NAPOR

Gene Symbol

CELF1

Additional CUGBP1/CELF1 Products

Product Documents for CUGBP1/CELF1 Antibody (7A1-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CUGBP1/CELF1 Antibody (7A1-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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