Cullin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00008454-D01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CUL1 Antibodies »

Product Specifications

Immunogen

CUL1 (NP_003583.2, 1 a.a. - 776 a.a.) full-length human protein. MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Cullin 1 Antibody

Western Blot: Cullin 1 Antibody [H00008454-D01P]

Western Blot: Cullin 1 Antibody [H00008454-D01P]

Western Blot: Cullin 1 Antibody [H00008454-D01P] - Analysis of CUL1 expression in transfected 293T cell line by CUL1 polyclonal antibody.Lane 1: CUL1 transfected lysate(89.70 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody [H00008454-D01P]

Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody [H00008454-D01P]

Immunocytochemistry/Immunofluorescence: Cullin 1 Antibody [H00008454-D01P] - Analysis of antibody to CUL1 on HeLa cell. Antibody concentration 30 ug/ml.
Proximity Ligation Assay: Cullin 1 Antibody [H00008454-D01P]

Proximity Ligation Assay: Cullin 1 Antibody [H00008454-D01P]

Proximity Ligation Assay: Cullin 1 Antibody [H00008454-D01P] - Analysis of protein-protein interactions between CUL1 and FBXW11. HeLa cells were stained with anti-CUL1 rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for Cullin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for WB.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CUL1

Cullin 1 may be involved in the degradation of positive cell cycle regulators (for example G1 cyclins in yeast). Human cullin 1 forms a complex with Skp1 and the F box protein Skp2, which is thought to play a role in the ubiquitination of G1 regulatory proteins, as its homolog does in yeast.

Long Name

Cullin 1

Alternate Names

Cullin 1

Gene Symbol

CUL1

UniProt

NP_003583.2 (Human)

Additional CUL1 Products

Product Documents for Cullin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cullin 1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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