Cullin 1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16092
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cullin 1 Antibody - Azide and BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 435-593 of human Cullin 1 (NP_003583.2). KNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTN
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cullin 1 Antibody - Azide and BSA Free
![Western Blot: Cullin 1 AntibodyAzide and BSA Free [NBP3-16092]](https://resources.bio-techne.com/images/products/Cullin-1-Antibody-Western-Blot-NBP3-16092-img0002.jpg "Western Blot: Cullin 1 AntibodyAzide and BSA Free [NBP3-16092]")
Western Blot: Cullin 1 AntibodyAzide and BSA Free [NBP3-16092]
Western Blot: Cullin 1 Antibody [NBP3-16092] - Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (NBP3-16092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092]](https://resources.bio-techne.com/images/products/Cullin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16092-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092]")
Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092]
Immunocytochemistry/Immunofluorescence: Cullin 1 Antibody [NBP3-16092] - Immunofluorescence analysis of HeLa cells using Cullin 1 antibody (NBP3-16092). Blue: DAPI for nuclear staining.
IHC-P- Cullin 1 Antibody [NBP3-16092] -
IHC-P- Cullin 1 Antibody [NBP3-16092] - Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for Cullin 1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:100
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CUL1
Long Name
Cullin 1
Alternate Names
Cullin 1
Gene Symbol
CUL1
Additional CUL1 Products
Product Documents for Cullin 1 Antibody - Azide and BSA Free
Product Specific Notices for Cullin 1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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