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Cullin 3 Antibody (7W3E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15821

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15821-100ul
NBP3-15821-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7W3E3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Cullin 3 Antibody (7W3E3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 669-768 of human Cullin 3 (Q13618). LHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cullin 3 Antibody (7W3E3)

Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821]

Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821]

Western Blot: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (NBP3-15821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Cullin 3 Antibody (7W3E3) [NBP3-15821]

Immunohistochemistry-Paraffin: Cullin 3 Antibody (7W3E3) [NBP3-15821]

Immunohistochemistry-Paraffin: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry of paraffin-embedded mouse brain using Cullin 3 antibody (NBP3-15821) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Cullin 3 Antibody (7W3E3) [NBP3-15821]

Immunohistochemistry-Paraffin: Cullin 3 Antibody (7W3E3) [NBP3-15821]

Immunohistochemistry-Paraffin: Cullin 3 Antibody (7W3E3) [NBP3-15821] - Immunohistochemistry of paraffin-embedded human oophoroma using Cullin 3 antibody (NBP3-15821) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Cullin 3 Antibody (7W3E3)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CUL3

Long Name

Cullin 3

Alternate Names

KIAA0617, NEDAUS, PHA2E

Gene Symbol

CUL3

Additional CUL3 Products

Product Documents for Cullin 3 Antibody (7W3E3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cullin 3 Antibody (7W3E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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